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KMID : 1094720110160051027
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Biotechnology and Bioprocess Engineering
2011 Volume.16 No. 5 p.1027 ~ p.1027
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Production of a thermostable metal-tolerant laccase from Trametes versicolor and its application in dye decolorization
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Zhu Youshuang
Zhang Haibo
Cao Mingle
Wei Zhenzhen
Huang Feng
Gao Peiji
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Abstract
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Production of laccase using a submerged culture of Trametes versicolor sdu-4 was optimized using a central composite design of the Response Surface Methodology. Optimized conditions gave a laccase yield of 4,213 U/L which was approximately three times of that in basal medium. The laccase was purified to homogeneity using a three-step process. The overall yield of the purification was 58%, with a purification fold of 11.4 and a specific activity of 1320.7 U/mg protein. The molecular mass of the laccase was 60 kDa. The optimum pH values of the enzyme were 2.2, 3.7, and 7 for the oxidations of ABTS, DMP, and syringaldazine, respectively. The enzyme had adaptability to a broad pH range and high temperature and wsa stable at pH 3.0 ∼ 10.0. The half-life of this laccase at 70°C was 2.2 h. Methyl red, 2-bromophenol, and 4-bromophenol were oxidized by the purified laccase in the absence of mediators. Purified laccase was effective in the decolorization of several dyes and was not inhibited by Cu2+, Mn2+, Zn2+, Na+, K+, Mg2+, Ba2+, and Ca2+ at 5 mM. These excellent characteristics made it a highly attractive candidate for industrial use.
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KEYWORD
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dye decolorization, laccase, response surface methodology, thermostable, trametes versicolor
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